Rabbit anti-HIF1-alpha Recombinant Monoclonal Antibody [BL-124-3F7] - BSA-Free
Product Details
Specifications
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
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HIF1-alpha [BL-124-3F7] was raised against a recombinant protein corresponding to residues 433-826 of Human Hypoxia-inducible factor 1-alpha isoform 1 using the numbering given in entry NP_001521.1 or Q16665 (GeneID 3091).
Additional Product Information
Hypoxia-inducible factor-1 (HIF1) is a basic helix-loop-helix (bHLH)-PER-ARNT-sim (PAS) transcription factor that plays a role in the cellular response to hypoxia. HIF1 is a heterodimer of an HIF1-alpha and HIF1-beta subunit. During normoxia, HIF-1alpha is regulated by ubiquitination and interaction with the von-Hippel-Lindau tumor suppressor protein (pVHL).
Alternate Names
ARNT-interacting protein;basic-helix-loop-helix-PAS protein MOP1;bHLHe78;class E basic helix-loop-helix protein 78;HIF1;HIF-1A;HIF-1alpha;HIF-1-alpha;HIF1-ALPHA;hypoxia inducible factor 1 alpha subunit;hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor);hypoxia-inducible factor 1 alpha isoform I.3;hypoxia-inducible factor 1-alpha;hypoxia-inducible factor1alpha;member of PAS protein 1;member of PAS superfamily 1;MOP1;PAS domain-containing protein 8;PASD8
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.