Rabbit anti-UBQLN4/CIP75/Ubiquilin 4 Recombinant Monoclonal Antibody [BLR145J]
Product Details
Specifications
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
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Immunogen was a recombinant protein representing human Ubiquilin-4 (residues 2-601, NP_064516.2).
Additional Product Information
Ubiquilin-4 (UBQLN4) plays a role in the regulation of protein degradation via the ubiquitin-proteasome system (UPS). It mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding (via UBA domain) to their polyubiquitin chains and by interacting (via ubiquitin-like domain) with the subunits of the proteasome. UBQLN4 Plays a role in the regulation of the proteasomal degradation of non-ubiquitinated GJA1 (By similarity). It acts as an adapter protein that recruits UBQLN1 to the autophagy machinery and mediates the association of UBQLN1 with autophagosomes and the autophagy-related protein LC3 (MAP1LC3A/B/C) and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion (PubMed:23459205) [taken from NCBI Entrez Gene (Gene ID: 56893)].
Alternate Names
A1U; A1Up; ataxin-1 interacting ubiquitin-like protein; ataxin-1 ubiquitin-like interacting protein; ataxin-1 ubiquitin-like-interacting protein A1U; C1orf6; CIP75; connexin43-interacting protein of 75 kDa; UBIN; ubiquilin-4
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.