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Goat anti-Rabbit Light Chain Antibody HRP Conjugated
Bethyl Laboratories
Catalog #
A120-113P
Target:
Rabbit Light Chain
Reactivity:
Rabbit
Applications:
WB
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
HRP
Purity:
Antigen Affinity Purified
Product Details
Specifications
Verified Reactivity
Rabbit
Antigen Species
Rabbit
Concentration
1 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
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Production Procedures
Antiserum was solid phase adsorbed to ensure specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit light chain. This antibody may cross react with other species.
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit light chain. This antibody may cross react with other species.
Country of Origin
USA
Additional Product Information
IgG is the primary immunoglobulin protein found in blood/plasma, and functions to neutralize toxins, active complement, immobilizing pathogens and inducing opsonization, and cell-mediated cytotoxicity. Anti-light chain antibodies are manufactured specifically to interact with the light chain of the IgG and excluded antibodies able to bind to the heavy chain.
Applications
Not all listed applications have been specifically tested by our laboratory.