ELISA Starter Accessory Kit (contains BSA blocking buffer)
Catalog #
ELISA
Product Details
2 - 8 °C
25 °C
25 °C or 2 - 8 °C
1 year from date of receipt
USA
Microtiter Wells Nunc MaxiSorp C bottom well Modules & Frames, #445101, 10 x 96 well plates
Store at room temperature in an area free from dust or debris.
ELISA Coating Buffer Carbonate-bicarbonate buffer capsules; Sigma Chemical #C3041, Package of 2
Store at room temperature for up to one year.
ELISA Wash Solution 50 mM Tris buffered saline, pH 8.0, 0.05% Tween 20; Sigma Chemical # T9039, 5 Packets
Store between 2 - 30 °C for up to one year.
ELISA Blocking Buffer 50 mM Tris buffered saline, pH 8.0, 1% BSA; Sigma Chemical # T6789, 1 Packet
Store between 2 - 8 °C for up to one year.
10% Tween 20 10% solution of polyoxyethylenesorbitan monolaurate (Tween 20; Sigma Product P7949
Store at room temperature for up to one year.
Enzyme Substrate TMB One Component HRP Microwell Substrate, 2 x 60 ml bottles
Store bottles between 2 - 8 °C for up to one year.
Microtiter Wells Nunc MaxiSorp C bottom well Modules & Frames, #445101, 10 x 96 well plates
Store at room temperature in an area free from dust or debris.
ELISA Coating Buffer Carbonate-bicarbonate buffer capsules; Sigma Chemical #C3041, Package of 2
Store at room temperature for up to one year.
ELISA Wash Solution 50 mM Tris buffered saline, pH 8.0, 0.05% Tween 20; Sigma Chemical # T9039, 5 Packets
Store between 2 - 30 °C for up to one year.
ELISA Blocking Buffer 50 mM Tris buffered saline, pH 8.0, 0.05% Tween20; Sigma Chemical # T9039, 2 Packets
Store between 2 - 8 °C for up to one year.
Enzyme Substrate TMB One Component HRP Microwell Substrate, 2 x 60 ml bottles
Store bottles between 2 - 8 °C for up to one year.
Tris Buffered Saline with 1% BSA, pH 8.0. Sigma Chemical #T-6789, 1 packet (makes 1 liter)
Tris Buffered Saline with Tween 20, pH 8.0. Sigma Chemical #T-9039, 1 packet (makes 1 liter)
Carbonate-bicarbonate buffer capsules, Sigma Chemical #C3041. Package of 2 (makes 200 ml)
10% solution of polyoxyethylenesorbitan monolaurate (Tween 20; Sigma Chemical #P7949).
ELISA Stop Solution contains 100 ml of 0.18 M H2SO4
Applications
Prepared Tris buffered saline with 1% BSA, pH 8.0, is suitable for use as a postcoat solution in ELISA.
Prepared Tris buffered saline with 1% BSA, pH 8.0, plus 0.05% Tween 20 (0.5 ml of 10% Tween 20 per 100 ml of postcoat) is suitable for use as sample and conjugate diluent.
Prepared Tris buffered saline with Tween 20, pH 8.0, is suitable for use as a wash solution in ELISA. Prepared carbonate-bicarbonate buffer is suitable for use in protein coating procedures on multiwall plates. 10% Tween 20 is suitable for use in ELISA wash buffers and diluents. ELISA Stop Solution is suitable to stop the TMB enzyme substrate reaction in ELISA.