Rabbit anti-AMBRA1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-568A maps to a region between residue 1200 and 1250 of human autophagy/beclin-1 regulator 1 using the numbering given in entry BAG64009.1 (GeneID 55626).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to AMBRA1 immobilized on solid support.
The epitope recognized by A302-568A-T maps to a region between residue 1200 and 1250 of human autophagy/beclin-1 regulator 1 using the numbering given in entry BAG64009.1 (GeneID 55626).
Additional Product Information
AMBRA1 (activating molecule in Beclin-1-regulated autophagy protein 1) is a protein that bears a WD40 domain and regulates Beclin-1-dependent autophagy. In mouse embryos AMBRA1 has been shown to be critical for neural tube development.
Alternate Names
activating molecule in beclin-1-regulated autophagy;activating molecule in BECN1-regulated autophagy protein 1;autophagy/beclin-1 regulator 1;DCAF3;DDB1 and CUL4 associated factor 3;WD repeat domain 94;WDR94
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.