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Rabbit anti-CD47 Recombinant Monoclonal Antibody [BLR131H] - BSA-Free

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
CD47
Reactivity:
Human
Platforms:
Simple Western
Host:
Rabbit
Clonality:
Recombinant Monoclonal
Clone:
BLR131H
Format:
Whole IgG
Immunogen:
Residues 19-142 (ECD)
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Purified
100 µg (BSA-Free)

100 µl

20 µl

$136.00 $851.00
Qty:

Product Details

Specifications

Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml 50 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Request Formulation Change
Production Procedures
Recombinant antibody was purified from cell culture supernatant.

Immunogen was a recombinant protein representing the extracellular domain of human leukocyte surface antigen CD47 (residues 19-142, NP_001768.1).
Country of Origin
USA

Additional Product Information

CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. CD47 is a receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q08722].

961
CD47
CD47 molecule
Q08722
Leukocyte surface antigen CD47

Alternate Names

antigen identified by monoclonal antibody 1D8;antigenic surface determinant protein OA3;CD antigen CD47;CD47 antigen (Rh-related antigen, integrin-associated signal transducer);CD47 glycoprotein;IAP;integrin associated protein;Integrin-associated protein;integrin-associated signal transducer;leukocyte surface antigen CD47;MER6;OA3;Protein MER6;Rh-related antigen

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).