Rabbit anti-BCKDHA, Phospho (S292) Recombinant Monoclonal Antibody [BLR060F] - BSA-Free
Product Details
Specifications
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
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Immunogen was a peptide surrounding phosphorylated serine 292 of mitochondrial human Branched Chain Keto Acid Dehydrogenase E1, Alpha polypeptide.
Using the numbering given for the precursor protein of entry NP_000700.1 (GeneID 593), the phosphorylated serine corresponds to residue 337.
Additional Product Information
BCKDHA (branched chain keto acid dehydrogenase E1, alpha polypeptide) is a component of the branched-chain alpha-keto acid (BCAA) dehydrogenase (BCKD) complex. The BCKD complex is an inner mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). Mutations in this gene result in maple syrup urine disease, type IA [taken from NCBI Entrez Gene (Gene ID: 593)].
Alternate Names
2-oxoisovalerate dehydrogenase (lipoamide);2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial;BCKDE1A;BCKDH E1-alpha;branched chain keto acid dehydrogenase E1 alpha protein;branched-chain alpha-keto acid dehydrogenase E1 component alpha chain;MSU;MSUD1;OVD1A
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).