Rabbit anti-PIK3R2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-594A-T maps to a region between residue 125 and 175 of human phosphoinositide-3-kinase, regulatory subunit 2 using the numbering given in entry NP_005018.1 (GeneID 5296).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PIK3R2 immobilized on solid support.
The epitope recognized by A302-594A maps to a region between residue 125 and 175 of human phosphoinositide-3-kinase, regulatory subunit 2 using the numbering given in entry NP_005018.1 (GeneID 5296).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Phosphoinositide-3-kinase regulatory subunit 2 (PIK3R2) binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O00459].
Alternate Names
MPPH;MPPH1;p85;P85B;p85-BETA;phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta;phosphatidylinositol 3-kinase regulatory subunit beta;phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta);phosphoinositide-3-kinase regulatory subunit beta;phosphoinositide-3-kinase, regulatory subunit 2 (beta);PI3K regulatory subunit beta;PI3-kinase regulatory subunit beta;PI3-kinase subunit p85-beta;PtdIns-3-kinase regulatory subunit beta;ptdIns-3-kinase regulatory subunit p85-beta
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.