Rabbit anti-Rubicon Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-569A maps to a region between residue 922 and 972 of human Rubicon using the numbering given in entry NP_055502.1 (GeneID 9711).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Rubicon immobilized on solid support.
The epitope recognized by A302-569A-T maps to a region between residue 922 and 972 of human Rubicon using the numbering given in entry NP_055502.1 (GeneID 9711).
Additional Product Information
Rubicon (RUN domain and cysteine-rich domain containing Beclin 1- interacting protein) was identified as a protein that associates with Beclin 1 and found to regulate endocytic trafficking and autophagy at the maturations step.
Alternate Names
baron; beclin-1 associated RUN domain containing protein; KIAA0226; RUBICON; RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein; run domain Beclin-1-interacting and cysteine-rich domain-containing protein; rundataxin; SCAR15
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.