Rabbit anti-Sequestosome-1 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
Sequestosome-1 is an adapter protein that binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. Sequestosome-1 may play a role in titin/TTN downstream signaling in muscle cells, and may regulate signaling cascades through ubiquitination. Sequestosome-1 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q13501].
Alternate Names
A170; DMRV; EBI3-associated protein of 60 kDa; EBI3-associated protein p60; EBIAP; FTDALS3; NADGP; OSIL; oxidative stress induced like; p60; p62; p62B; PDB3; phosphotyrosine independent ligand for the Lck SH2 domain p62; phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; sequestosome-1; ubiquitin-binding protein p62; ZIP3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.