Rabbit anti-Topo II Alpha Recombinant Monoclonal Antibody [BLR358N]
Product Details
Specifications
Request Formulation Change Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free
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Immunogen was a peptide representing a region between residue 650 and 700 of human DNA topoisomerase 2-alpha using the numbering given in entry NP_001058.2 (Gene ID 7153).
Additional Product Information
Topoisomerases regulate topological states of DNA and are divided into type I and type II enzymes. Type II topoisomerases make double stranded breaks in DNA and allow the passage of another duplex DNA across the break to achieve unknotting of entangled DNA. Topo II Alpha is one of two described isoforms that are likely products of a gene duplication event. TopoII Alpha is also described to be involved in chromosome condensation, chromatid separation, and the relief of supercoil stress.
Alternate Names
DNA gyrase; DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase 2-alpha; DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; TOP2; topoisomerase (DNA) II alpha 170kDa; TP2A
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).