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Rabbit anti-VMA21 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
VMA21
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 51 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Presumed Reactivity
Chicken, X. tropicalis, Zebrafish, Bovine
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to VMA21 immobilized on solid support.

The epitope recognized by A305-701A maps to a region between residue 51 to 101 of human Vacuolar ATPase assembly integral membrane protein VMA21 using the numbering given in entry Q3ZAQ7.1 (GeneID 203547).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to VMA21 immobilized on solid support.

The epitope recognized by A305-701A-T maps to a region between residue 51 to 101 of human Vacuolar ATPase assembly integral membrane protein VMA21 using the numbering given in entry Q3ZAQ7.1 (GeneID 203547).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

Vacuolar ATPase assembly integral membrane protein VMA21is a chaperone for assembly of lysosomal vacuolar ATPase [taken from NCBI Entrez Gene (Gene ID: 203547)].

203547
VMA21
VMA21, vacuolar ATPase assembly factor
Q3ZAQ7
Vacuolar ATPase assembly integral membrane protein VMA21

Alternate Names

MEAX; myopathy with excessive autophagy protein; vacuolar ATPase assembly integral membrane protein VMA21; VMA21 vacuolar H+-ATPase homolog; XMEA

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.