Rabbit anti-Vps15 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-570A maps to a region between residue 1075 and 1125 of human vacuolar protein sorting 15 using the numbering given in entry NP_055417.1 (GeneID 30849).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Vps15 immobilized on solid support.
The epitope recognized by A302-570A-T maps to a region between residue 1075 and 1125 of human vacuolar protein sorting 15 using the numbering given in entry NP_055417.1 (GeneID 30849).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
VPS (vacuolar protein sorting) proteins were originally characterized and studied in the yeast model and found to play a critical role in vesicle-mediated sorting of proteins to lysosome-like vacuoles. The human homolog of the yeast Vps15 protein was identified as a 150 kDa cellular protein that associates with the phosphatidylinositol (PtdIns) 3 kinase, Vps34p. Vps15 is a serine/threonine protein kinase that associates in a complex with beclin 1 and plays an important role in autophagy.
Alternate Names
p150; phosphatidylinositol 3-kinase-associated p150; phosphoinositide 3-kinase adaptor protein; phosphoinositide 3-kinase regulatory subunit 4; phosphoinositide-3-kinase, regulatory subunit 4, p150; PI3-kinase p150 subunit; PI3-kinase regulatory subunit 4; VPS15
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.