Rabbit anti-HdmX/MDM4 Recombinant Monoclonal Antibody [BL-3-2F2]
Product Details
Specifications
The epitope recognized by A700-000-T maps to a region between residues 125 and 175 of human ortholog of mouse double minute 4 using the numbering given in entry NP_002384.2 (GeneID 4194). Cell culture supernatant was harvested from mammalian cells expressing recombinant rabbit monoclonal antibody.
The epitope recognized by A700-000 maps to a region between residues 125 and 175 of human ortholog of mouse double minute 4 using the numbering given in entry NP_002384.2 (GeneID 4194).
Additional Product Information
HdmX/MDM4 is a RING-finger domain containing protein that interacts with p53. HdmX/MDM4 may influence p53 stability through an interaction with the structurally similar MDM2, an ubiquitin-ligase that targets p53 for proteasomal degradation. HdmX/MDM4 may influence p53 by directly attenuating p53 transcriptional activity.
Alternate Names
Double minute 4 protein; double minute 4, human homolog of; HDMX; mdm2-like p53-binding protein; Mdm4 p53 binding protein homolog; MDM4 protein variant G; MDM4 protein variant Y; MDM4-related protein 1; MDMX; MRP1; p53-binding protein; p53-binding protein Mdm4; protein Mdm4; protein Mdmx
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.