Rabbit anti-AMPK alpha 2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-508A maps to a region between residues 350 and 400 of human 5'-AMP-activated Protein Kinase, catalytic Alpha-2 chain using the numbering given in entry AAH69823.1 (GeneID 5563).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to AMPK alpha 2 immobilized on solid support.
The epitope recognized by A300-508A-T maps to a region between residues 350 and 400 of human 5'-AMP-activated Protein Kinase, catalytic Alpha-2 chain using the numbering given in entry AAH69823.1 (GeneID 5563).
Additional Product Information
AMP-activated protein kinase (AMPK) is a heterotrimeric protein made up of a catalytic alpha subunit, and a regulatory beta and gamma subunit. There are two distinct genes for the alpha subunit, alpha1 and alpha2. AMPK alpha 2 is the serine/threonine kinase catalytic subunit of the AMPK. AMPK is responsible for regulating fatty acid and cholesterol synthesis. AMPK is activated in response to a wide variety of metabolic stresses and switches off anabolic pathways when ATP levels are depleted.
Alternate Names
5'-AMP-activated protein kinase catalytic subunit alpha-2;5'-AMP-activated protein kinase, catalytic alpha-2 chain;ACACA kinase;acetyl-CoA carboxylase kinase;AMP-activated protein kinase alpha-2 subunit variant 2;AMP-activated protein kinase alpha-2 subunit variant 3;AMPK;AMPK alpha 2;AMPK subunit alpha-2;AMPK2;AMPKa2;AMPK-alpha-2 chain;HMGCR kinase;hydroxymethylglutaryl-CoA reductase kinase;PRKAA;protein kinase, AMP-activated, alpha 2 catalytic subunit
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.