Rabbit anti-Beclin 1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-567A maps to a region between residue 50 and 100 of human Beclin 1 using the numbering given in entry NP_003757.1 (GeneID 8678).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Beclin 1 immobilized on solid support.
The epitope recognized by A302-567A-T maps to a region between residue 50 and 100 of human Beclin 1 using the numbering given in entry NP_003757.1 (GeneID 8678).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Beclin 1 was initially identified as a Bcl-2 interacting protein. Beclin 1 has been found to play a role in autophagy, the process whereby cellular structures, organelles, and intracellular pathogens undergo degradation. As an interacting protein of Bcl-2, beclin is able to influence Bcl-2 to function as an autophagy protein.
Alternate Names
ATG6;ATG6 autophagy related 6 homolog;beclin 1 (coiled-coil, moesin-like BCL2-interacting protein);beclin 1, autophagy related;beclin1;beclin-1;coiled-coil myosin-like BCL2-interacting protein;Protein GT197;testis secretory sperm-binding protein Li 215e;VPS30
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.