Rabbit anti-CCNA2/Cyclin A2 Recombinant Monoclonal Antibody [BLR369N]
Product Details
Specifications
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Immunogen was a peptide representing a region between residue 382 and the C-terminus (residue 432) of human Cyclin A2 (aka CCNA2) using the numbering given in entry NP_001228.2 (Gene ID 890).
Additional Product Information
The protein encoded by the gene CCNA2, Cyclin A2, belongs to the highly conserved cyclin family. Members of the cyclin family are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, Cyclin A2 is expressed in all tissues tested. Cyclin A2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions [taken from NCBI Entrez Gene ID: 890].
Alternate Names
CCN1; CCNA; Cyclin A; cyclin-A; cyclin-A2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (A120-113P) with 5% Normal Pig Serum (S100-020) added to the blocking buffer.