Rabbit anti-RUNX2 Recombinant Monoclonal Antibody [BLR360N]
Product Details
Specifications
Request Formulation Change Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free
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Immunogen was a peptide representing a region between residue 300 and 350 of human Runt-related transcription factor 2 (RUNX2) using the numbering given in entry NP_001019801.3 (Gene ID 8
Additional Product Information
RUNX2 is a member of the RUNX family of transcription factors and encodes a nuclear protein with a Runt DNA-binding domain. RUNX2 is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. RUNX2 can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. [provided by RefSeq, Jul 2016] [taken from NCBI Entrez Gene (Gene ID: 860)].
Alternate Names
acute myeloid leukemia 3 protein;AML3;CBFA1;CBF-alpha-1;CCD;CCD1;CLCD;Core-binding factor subunit alpha-1;core-binding factor, runt domain, alpha subunit 1;oncogene AML-3;OSF2;OSF-2;osteoblast-specific transcription factor 2;PEA2aA;PEA2-alpha A;PEBP2aA;PEBP2-alpha A;polyomavirus enhancer-binding protein 2 alpha A subunit;runt-related transcription factor 2;SL3/AKV core-binding factor alpha A subunit;SL3-3 enhancer factor 1 alpha A subunit
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (A120-113P) with 5% Normal Pig Serum (S100-020) added to the blocking buffer.