Rabbit anti-Vps15 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
VPS (vacuolar protein sorting) proteins were originally characterized and studied in the yeast model and found to play a critical role in vesicle-mediated sorting of proteins to lysosome-like vacuoles. The human homolog of the yeast Vps15 protein was identified as a 150 kDa cellular protein that associates with the phosphatidylinositol (PtdIns) 3 kinase, Vps34p. Vps15 is a serine/threonine protein kinase that associates in a complex with beclin 1 and plays an important role in autophagy.
Alternate Names
p150; phosphatidylinositol 3-kinase-associated p150; phosphoinositide 3-kinase adaptor protein; phosphoinositide 3-kinase regulatory subunit 4; phosphoinositide-3-kinase, regulatory subunit 4, p150; PI3-kinase p150 subunit; PI3-kinase regulatory subunit 4; VPS15
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.