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Rabbit anti-Vps15 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
Vps15
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1308 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

10 µl (200 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Presumed Reactivity
Rat
Antigen Species
Human
Concentration
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to Vps15 immobilized on solid support.

The epitope recognized by A302-571A-T maps to a region between residue 1300 and 1358 of human vacuolar protein sorting 15 using the numbering given in entry NP_055417.1 (GeneID 30849).
Antibody was affinity purified using an epitope specific to Vps15 immobilized on solid support.

The epitope recognized by A302-571A maps to a region between residue 1300 and 1358 of human vacuolar protein sorting 15 using the numbering given in entry NP_055417.1 (GeneID 30849).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

VPS (vacuolar protein sorting) proteins were originally characterized and studied in the yeast model and found to play a critical role in vesicle-mediated sorting of proteins to lysosome-like vacuoles. The human homolog of the yeast Vps15 protein was identified as a 150 kDa cellular protein that associates with the phosphatidylinositol (PtdIns) 3 kinase, Vps34p. Vps15 is a serine/threonine protein kinase that associates in a complex with beclin 1 and plays an important role in autophagy.

30849
PIK3R4
phosphoinositide-3-kinase regulatory subunit 4
Q99570
Phosphoinositide 3-kinase regulatory subunit 4

Alternate Names

p150; phosphatidylinositol 3-kinase-associated p150; phosphoinositide 3-kinase adaptor protein; phosphoinositide 3-kinase regulatory subunit 4; phosphoinositide-3-kinase, regulatory subunit 4, p150; PI3-kinase p150 subunit; PI3-kinase regulatory subunit 4; VPS15

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.