Rabbit anti-WRN Antibody, Affinity Purified
Product Details
Specifications
The epitope recognized by A300-238A maps to a region between residues 400 and 450 of human Werner Syndrome Helicase using the numbering given in SwissProt entry Q14191 (GeneID 7486).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to WRN immobilized on solid support.
The epitope recognized by A300-238A-T maps to a region between residues 400 and 450 of human Werner Syndrome Helicase using the numbering given in SwissProt entry Q14191 (GeneID 7486).
Additional Product Information
The protein product of the Werner’s syndrome (WRN) gene is the Werner syndrome ATP-dependent helicase. WRN is part of the ReqQ family of helicases that unwind DNA in preparation for replication, transcription, and repair. WRN appears to play a versatile role in cellular processes including DNA replication, transcription and the maintenance of genomic integrity.
Alternate Names
DNA helicase, RecQ-like type 3; exonuclease WRN; recQ protein-like 2; RECQ3; RECQL2; RECQL3; Werner syndrome ATP-dependent helicase; Werner syndrome, RecQ helicase-like
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.